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Both P25TiO2NPs (with or without vitamin B2) were not found beyond the epidermis in 99% of the analyzed TEM images (Fig. 8). This is coherent with previous findings showing that nanoparticles greater than 50 nm can not penetrate the skin, even in vivo models with movement, stretching, and friction [54]. However, in one of the zones, a few nanoparticles were observed inside a hair follicle. This could be due to the follicle exposure after the localized rupture of this physical barrier when rats were shaved in order to clean the area for cream topical administration. This finding suggests that nanoparticle-based sunscreen should not be applied on recently shaved or harmed skin, in order to avoid nanoparticle skin penetration.

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Moreover, the safety measures in these factories are paramount. Workers are equipped with protective gear and trained to handle potentially hazardous chemicals safely Workers are equipped with protective gear and trained to handle potentially hazardous chemicals safely Workers are equipped with protective gear and trained to handle potentially hazardous chemicals safely Workers are equipped with protective gear and trained to handle potentially hazardous chemicals safelyr 5566 titanium dioxide factories. Stringent regulations, such as OSHA standards in the US and EU REACH guidelines, ensure that these factories operate responsibly and ethically.

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